|
|
| Consortium for Maize Genomics |
|
|
|
Strategies: Methyl-Filtration
Methyl-filtration, a technique licensed to Orion Genomics, exploits the
finding that the majority of retrotransposon and repetitive sequences in maize are methylated.
Plant genomes are comprised of ISLANDS of genes nestled among OCEANS of repetitive junk DNA.
The repetitive DNA is comprised mostly of retrotransposon elements that are heavily methylated (red bars), while
genes (black arrows) are not methylated.
With the GeneThrsher® approach...
...genomic subclones are generated by fragmenting DNA from the plant of interest.
The resulting library primarily contains the genespace and reduces the size of complexity of the genome.
Go back to "Strategies"
|
|
|
|
